Soybean small RNA (Ribonucleic Acid) gene miR85-3p and application thereof in regulation and control of high temperature and drought of plants

The invention belongs to the technical field of gene engineering, and discloses application of a soybean small RNA (Ribonucleic Acid) gene miR85-3p in regulation and control of high temperature and drought of plants. Wherein the mature sequence of the soybean small RNA gene miR85-3p is as shown in S...

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Hauptverfasser: LIAO WEI, WANG HENG, TIAN ZHUO, YANG WANYING, WANG XIAOBO, ZHI XIANGUAN, CHEN HAORAN, LI JIAJIA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of gene engineering, and discloses application of a soybean small RNA (Ribonucleic Acid) gene miR85-3p in regulation and control of high temperature and drought of plants. Wherein the mature sequence of the soybean small RNA gene miR85-3p is as shown in SEQ ID NO. 1 in a sequence table; the sequence of the precursor gene of the soybean small RNA gene miR85-3p is as shown in SEQ ID NO. 2 in a sequence table. The soybean small RNA gene miR85-3p is applied to arabidopsis thaliana, the survival rate of the seedling stage of arabidopsis thaliana and the seed quality are negatively regulated and controlled by inhibiting expression of downstream target genes under high temperature stress, the germination rate of arabidopsis thaliana seeds under drought stress can be regulated and controlled, and the effect of inhibiting growth and development of arabidopsis thaliana under high temperature and drought stress is achieved. 本发明属于基因工程技术领域,公开了大豆小RNA基因miR85-3p在植物高温和干旱调控中的应用。其中,大