Application of arabidopsis HIPP32 gene in regulation and control of plant lead stress tolerance
The invention relates to an application of an arabidopsis HIPP32 gene in regulating and controlling the lead stress tolerance of a plant. According to the invention, a homozygous mutant of the arabidopsis HIPP32 gene is obtained through PCR (Polymerase Chain Reaction) identification; an HIPP32 gene...
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| creator | ZHOU SHUNKANG LIU TING XIE LIJUAN HUANG DONGWEI ZHENG YUPING QI HUA QIU RONGLIANG |
| description | The invention relates to an application of an arabidopsis HIPP32 gene in regulating and controlling the lead stress tolerance of a plant. According to the invention, a homozygous mutant of the arabidopsis HIPP32 gene is obtained through PCR (Polymerase Chain Reaction) identification; an HIPP32 gene overexpression vector is obtained through a gene engineering technology, an HIPP32 gene overexpression arabidopsis thaliana strain is obtained, and a homozygous arabidopsis thaliana strain with the HIPP32 gene overexpression is finally obtained after screening. The tolerance of the arabidopsis HIPP32 gene mutant to lead stress is enhanced, the resistance of an overexpressed plant to lead is weakened, and under lead treatment, the elongation and chlorophyll content of the HIPP32 gene mutant root are obviously higher than those of a wild plant, and the elongation and chlorophyll content of the overexpressed plant root are obviously lower than those of the wild plant. According to the invention, the effect of the arab |
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According to the invention, a homozygous mutant of the arabidopsis HIPP32 gene is obtained through PCR (Polymerase Chain Reaction) identification; an HIPP32 gene overexpression vector is obtained through a gene engineering technology, an HIPP32 gene overexpression arabidopsis thaliana strain is obtained, and a homozygous arabidopsis thaliana strain with the HIPP32 gene overexpression is finally obtained after screening. The tolerance of the arabidopsis HIPP32 gene mutant to lead stress is enhanced, the resistance of an overexpressed plant to lead is weakened, and under lead treatment, the elongation and chlorophyll content of the HIPP32 gene mutant root are obviously higher than those of a wild plant, and the elongation and chlorophyll content of the overexpressed plant root are obviously lower than those of the wild plant. 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According to the invention, a homozygous mutant of the arabidopsis HIPP32 gene is obtained through PCR (Polymerase Chain Reaction) identification; an HIPP32 gene overexpression vector is obtained through a gene engineering technology, an HIPP32 gene overexpression arabidopsis thaliana strain is obtained, and a homozygous arabidopsis thaliana strain with the HIPP32 gene overexpression is finally obtained after screening. The tolerance of the arabidopsis HIPP32 gene mutant to lead stress is enhanced, the resistance of an overexpressed plant to lead is weakened, and under lead treatment, the elongation and chlorophyll content of the HIPP32 gene mutant root are obviously higher than those of a wild plant, and the elongation and chlorophyll content of the overexpressed plant root are obviously lower than those of the wild plant. 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According to the invention, a homozygous mutant of the arabidopsis HIPP32 gene is obtained through PCR (Polymerase Chain Reaction) identification; an HIPP32 gene overexpression vector is obtained through a gene engineering technology, an HIPP32 gene overexpression arabidopsis thaliana strain is obtained, and a homozygous arabidopsis thaliana strain with the HIPP32 gene overexpression is finally obtained after screening. The tolerance of the arabidopsis HIPP32 gene mutant to lead stress is enhanced, the resistance of an overexpressed plant to lead is weakened, and under lead treatment, the elongation and chlorophyll content of the HIPP32 gene mutant root are obviously higher than those of a wild plant, and the elongation and chlorophyll content of the overexpressed plant root are obviously lower than those of the wild plant. According to the invention, the effect of the arab</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Application of arabidopsis HIPP32 gene in regulation and control of plant lead stress tolerance |
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