Process for preparing pig-derived high-purity chymotrypsin by using composite anion chromatography method

The invention relates to the technical field of chymotrypsin preparation, and provides a process for preparing pig-derived high-purity chymotrypsin by using a composite anion chromatography method, and the process comprises the following steps: S1, extraction: obtaining pig pancreas, and carrying out mashing treatment; s2, acid leaching: performing acid leaching on the crushed pancreas by using sulfuric acid; s3, filtering and clarifying: centrifugally filtering the solution formed in S2, and clarifying by using a diatomite filter aid; S4, salting out: adding ammonium sulfate into the solution formed in S3, and collecting ammonium sulfate saturation precipitate; s5, dissolving and activating: adding a phosphate buffer solution into the precipitate formed in the step S4, and adjusting the pH value; s6, dilution: diluting the solution formed in the step S5 by using a phosphate buffer solution; s7, carrying out pre-balancing; s8, loading a sample; s9, carrying out post-balancing; s10, washing impurities; and S11