Glutamine transaminase with improved thermal stability and application thereof

The invention discloses glutamine transaminase with improved thermal stability and application thereof, and belongs to the fields of biology and food. Site-specific mutagenesis is carried out on the basis of a female parent TGase, and compared with glutamine transaminase shown in SEQ ID NO.1, the ha...

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Hauptverfasser:	YANG PENGHUI, LIU SONG, CHEN JIAN, PLUGOC CHENG, QIU WENXUAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses glutamine transaminase with improved thermal stability and application thereof, and belongs to the fields of biology and food. Site-specific mutagenesis is carried out on the basis of a female parent TGase, and compared with glutamine transaminase shown in SEQ ID NO.1, the half-life periods at 60 DEG C, 65 DEG C and 70 DEG C of the obtained glutamine transaminase variant FRAPD-TGm2C are increased by 6.5 times, 7.2 times and 6.5 times respectively. In addition, the specific enzyme activities at 70 DEG C, 75 DEG C and 80 DEG C are respectively 2.95 times, 2.33 times and 1.31 times of the specific enzyme activities of the glutamine transaminase shown as SEQ ID NO.1, and the casein cross-linking activity of the glutamine transaminase shown as SEQ ID NO.1 at the high temperature of 78 DEG C is obviously higher than that of the glutamine transaminase shown as SEQ ID NO.1. The TGase variant with improved thermal stability constructed by the invention is more beneficial to stable application o