Preparation method and application of vitamin B3 deficiency model based on liver primary cells

The invention belongs to a bioengineering technology, and discloses a preparation method and application of a vitamin B3 deficiency model based on liver primary cells. The method comprises the following steps: (1) extracting primary liver cells, planting the primary liver cells on a culture dish by using a DMEM (Dulbecco Modified Eagle Medium) (containing serum) at the planting density of 5 * 10 < 5 >/mL, and replacing the DMEM (Dulbecco Modified Eagle Medium) (containing serum) after planting for about 5-6 hours; (2) changing the culture medium next day, and changing a vitamin B3 deficiency culture medium (serum-free) in the hepatocyte deficiency model building group; (3) on the third day, constructing a hepatocyte deficiency model and replacing a vitamin B3 deficiency culture medium (serum-free); and (4) on the fourth day, replacing the vitamin B3 deficiency culture medium (serum-free) with the hepatocyte deficiency model construction group, and continuously culturing. The model construction success rate ca