Phosphate bionic hydrolase capable of being regulated and controlled by light as well as preparation method and application of phosphate bionic hydrolase

The invention discloses a light-controllable phosphate bionic hydrolase as well as a preparation method and application thereof, a catalytic substrate 2-hydroxypropyl (4-nitrophenyl) sodium phosphate (HPNP) is hydrolyzed to generate p-nitrophenol anions, and a strong absorption peak is formed at the wavelength of 400nm. An azobenzene type catalyst Azo-C7-TACN with a photosensitive characteristic is synthesized from a 1, 4, 7-triaza-nonyl alkyl group (TACN), and after the azobenzene type catalyst Azo-C7-TACN is coordinated with Zn < 2 + >, the influence of conversion of a light-operated configuration of the azobenzene type catalyst Azo-C7-TACN on the hydrolysis reaction activity of a catalytic substrate HPNP is researched. The configuration of the Azo-C7-TACN-Zn < 2 + > can be switched between a cis form and a trans form, can be repeatedly converted and has repeatability, and the catalytic efficiency k2 of the Azo-C7-TACN-Zn < 2 + > of the E configuration on HPNP hydrolysis reaction is higher than that of the