Chicken semen low-temperature preservation diluent as well as preparation method and application thereof

The invention discloses a diluent for low-temperature preservation of chicken seminal fluid and a preparation method and application thereof, and belongs to the technical field of poultry breeding, the diluent for low-temperature preservation of chicken seminal fluid is prepared from the following raw materials: 1.2-1.6 g of glucose, 1.1-1.5 g of sodium glutamate, 2.2-2.6 g of dipotassium phosphate, 0.34-0.38 g of monopotassium phosphate, 0.03-0.07 g of potassium citrate, 0.03-0.07 g of magnesium chloride, 0.05-0.15 g of EDT A, 5-15 [mu] L of an antibiotic solution, and 80-120 mL of ultrapure water. The preparation method comprises the following steps: weighing raw materials, mixing with ultrapure water, stirring, filtering with a filter, and mixing with an antibiotic solution to obtain a diluent; the chicken semen diluent provides a suitable living environment for the chicken semen preserved in vitro in a liquid state, prolongs the in vitro preservation time of the chicken semen, and improves the in vitro fe