Primer pair and kit for detecting monkey pox virus based on recombinant polymerase amplification and CRISPR/Cas12a

The invention belongs to the technical field of virus detection, and particularly relates to a primer pair and a kit for detecting monkey pox virus based on recombinant polymerase amplification and CRISPR/Cas12a. The sequence of the recombinant polymerase amplification primer pair disclosed by the i...

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Hauptverfasser:	WANG MIN, ZHAO YINZHEN, LI LINGKE, CHENG LEI, WANG JICHUANG, WANG XINGLAN, ZHANG YIQING, LI YULIN, NIU NANNAN, DU XUELI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of virus detection, and particularly relates to a primer pair and a kit for detecting monkey pox virus based on recombinant polymerase amplification and CRISPR/Cas12a. The sequence of the recombinant polymerase amplification primer pair disclosed by the invention is as shown in SEQ ID NO.1-2. According to the invention, a novel detection technology combining CRISPR/Cas12a with RPA and lateral immunochromatography is established; a new rapid detection technology for the monkey pox virus is established, and compared with a traditional PCR detection method, the detection time of the detection method established by the project is shortened by at least 1 h. 本发明属于病毒检测技术领域，尤其涉及一种基于重组聚合酶扩增和CRISPR/Cas12a检测猴痘病毒的引物对和试剂盒。本发明所述重组聚合酶扩增引物对的序列如SEQ ID NO.1～2所示。本发明建立CRISPR/Cas12a结合RPA及侧向免疫层析的检测新技术；建立针对猴痘病毒的快速检测新技术，本项目建立的检测方法相比于传统PCR检测方法的检测时间至少缩短1h。