Annular fluorescent probe mediated isothermal amplification system based on micro-fluidic chip, kit and application

The invention discloses an annular fluorescent probe mediated isothermal amplification system based on a micro-fluidic chip, a kit and application, can realize multi-target and high-throughput detection of pathogenic vibrio parahaemolyticus in prawns, and has a very good application prospect in the field of rapid detection of food-borne pathogenic bacteria. According to the method, the target DNA is specifically marked by virtue of the annular fluorescent probe, so that the problems that non-specific amplification is easy to occur, false positive signals are generated and the like in a conventional isothermal nucleic acid amplification method are solved; multi-channel on-site detection is realized by virtue of a micro-fluidic chip, and the limitation that the existing method is limited by a site and cannot realize on-site rapid detection is solved. 本发明公开了基于微流控芯片的环形荧光探针介导的等温扩增体系、试剂盒及应用，可实现对虾中致病性副溶血性弧菌的多靶标、高通量检测，在食源性致病菌快速检测领域具有非常好的应用前景。本发明借助环形荧光探针对靶标DNA进行特异性标记，解决常规等温核酸扩增方法中容易出现非特异性扩增、产生假阳性信号等问题；借助微流控芯片实现多通道现场检测