Macrophage function screening system and screening method and application thereof

The invention discloses a macrophage function screening system as well as a screening method and application thereof. The macrophage function screening system comprises a CRISPR-dcas9 system and a suicide gene system, wherein the CRISPR-dcas9 system is used for overexpressing a gene of an sgRNA library and changing the polarization state and function of macrophages; the suicide gene system is used for screening polarization states and functions of macrophages. According to the method, a polarization signal of the macrophages is converted into a signal of the number of the cells, so that possibility is provided for screening polarization of the macrophages, key polarized genes are screened in a genome library, and regulated genes are more comprehensively screened; therefore, compared with the existing gene, a more complete and key regulatory gene bank is obtained and is used for treating subsequent diseases. 本发明公开了一种巨噬细胞功能筛选系统及其筛选方法和应用，包括CRISPR-dcas9系统和自杀基因系统；其中：所述CRISPR-dcas9系统，用于过表达sgRNA文库的基因，改变巨噬细胞的极化状态与功能；