Comprehensive analysis method for searching peripheral nerve infringement markers of patients with head and neck malignant tumors

The invention discloses a comprehensive analysis method for finding a marker for finding whether a patient with head and neck malignant tumors has peripheral nerve invasion or not, and belongs to the technical field of biology. The method comprises the following steps: collecting original RNA sequencing data and clinical features from a certain TCGA cancer species queue, and then dividing the original RNA sequencing data and clinical features into a PNI group and a non-PNI group; analyzing differentially expressed genes in the PNI group and the non-PNI group by using the R packet DESeq2, so as to correct the post Plt; the absolute value gt of the difference multiple is 0.05; 2 is used as a threshold value for screening differential genes; carrying out GO and KEGG pathway enrichment analysis on the differential genes; analyzing the differential genes by using minimum absolute contraction and selection operator (LASSO) regression analysis; the most key PNI related genes are obtained from LASSO analysis; calcula