Central obesity resistance marker as well as detection method and application thereof

The invention discloses a BDNF protein detection kit, the kit comprises a calibrator, a capture antibody, a detection antibody and a matched detection reagent, the detection antibody is a monoclonal antibody coupled with alkaline phosphatase, the monoclonal antibody can specifically bind to a mouse BDNF protein and also can specifically bind to a human BDNF protein, and the detection reagent is used for detecting the BDNF protein. Amino acid sequences of a heavy chain variable region and a light chain variable region of the monoclonal antibody are shown as SEQ ID NO.2 and SEQ ID NO.3. The invention further discloses a preparation method of the monoclonal antibody. On the basis of the screened monoclonal antibody, the kit for detecting the BDNF protein is established in combination with other reagents, and the kit has good sensitivity and specificity, can be used for specifically detecting the BDNF protein of mice and the BDNF protein of human, and can be used for detecting the BDNF protein of the mice and the