Aaquaporin determination method based on metal nanoprobe labeling-inductively coupled plasma mass spectrometry detection strategy

The invention belongs to the technical field of protein detection, and particularly discloses an aquaporin determination method based on a metal nanoprobe labeling-inductively coupled plasma mass spectrometry detection strategy, which utilizes the reaction activity of an epoxy group on the surface of a magnetic bead with amino and sulfydryl to realize the combination of the magnetic bead and aquaporin AQP1; by utilizing the characteristic of specific recognition of an antigen and an antibody, antibody protein is coupled to the surface of a metal nanoprobe, and aquaporin AQP1 is fixed on the surface of a magnetic bead, so that the aquaporin AQP1 and a corresponding amount of metal probe are fixed on the magnetic bead, and specific labeling of the aquaporin AQP1 is realized; and converting the amount of aquaporin AQP1 into the amount of a metal probe; after the redundant metal probe is washed away, a citric acid elution method is used for releasing the metal probe, quantitative analysis is carried out on the me