OTA analysis method based on efficient homogeneous electrochemiluminescence aptamer sensor

The invention discloses an OTA analysis method based on an efficient homogeneous electrochemiluminescence aptamer sensor. The OTA analysis method comprises the following steps: preparing a mixed solution of L/S *-Ru, IA/B, a hairpin probe H1, a hairpin probe H2 and exonuclease Exo-III; oTA is put into the mixed solution for a reaction, and a reaction solution is obtained; and pouring the reaction solution into a solution containing 0.02 M of TPrA to obtain a test solution, immersing an electrode into the test solution to carry out ECL detection, and reading a detection result. The analysis method has the advantages of high stability, high sensitivity and good selectivity, and can be used for detecting other biomolecules such as toxins and proteins by changing the aptamer sequence and the blocking chain. 公开了一种基于高效均相电致化学发光适配体传感器的OTA分析方法，包括配制L/S*-Ru、IA/B、发夹探针H1、发夹探针H2和核酸外切酶Exo-III混合液；将OTA投入混合液中进行反应，得到反应溶液；将反应溶液倒入含0.02M TPrA的溶液中，得到测试溶液，将电极浸入测试溶液中进行ECL检测并读取检测结果。该分析方法具有稳定性高、灵敏度高以及选择性好的优点，同时，通过改变适配体序列和阻断链能够用于检测其他毒素、