Preparation of bifunctional fluorescence sensor and application of bifunctional fluorescence sensor in SARS-CoV-2 Mpro detection and inhibitor screening

Preparation of bifunctional fluorescence sensor and application of bifunctional fluorescence sensor in SARS-CoV-2 Mpro detection and inhibitor screening

The invention discloses preparation of a difunctional fluorescence sensor and application of the difunctional fluorescence sensor in SARS-CoV-2Mpro detection and inhibitor screening, and the preparation comprises the following steps: dissolving an AIE probe TPE-Ph-In and a substrate S-TLG in a THF-H...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: YU XIEAN, WANG PING, WANG BING, HAN LEI, LIN JIAOLI, LI MEIFANG, TIAN JIANGWEI
Format: Patent
Sprache:chi ; eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator YU XIEAN
WANG PING
WANG BING
HAN LEI
LIN JIAOLI
LI MEIFANG
TIAN JIANGWEI
description The invention discloses preparation of a difunctional fluorescence sensor and application of the difunctional fluorescence sensor in SARS-CoV-2Mpro detection and inhibitor screening, and the preparation comprises the following steps: dissolving an AIE probe TPE-Ph-In and a substrate S-TLG in a THF-H2O solution, fully and uniformly mixing, adding glutaraldehyde, and stirring for reaction under closed and dark conditions; the fluorescence sensor is obtained after the reaction is completed. The sensor disclosed by the invention is simple to prepare, mild in reaction condition, convenient to operate and easy to prepare in batches, and a response result can be obtained within 45 minutes after the constructed sensor is added into SARS-CoV-2Mpro to be incubated: after the anti-Mpro active substance is added, different fluorescence signals are generated and can reflect the strength of the anti-Mpro activity, so that the sensitivity of the anti-Mpro activity is improved. And a new tool and a new method are provided fo
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN118533798A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN118533798A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN118533798A3</originalsourceid><addsrcrecordid>eNqNjUEKwjAQRbtxIeodxgN0UYvYLktR3ChixW2ZphMdCJOQpHfxuNbiAbr6fHjv_2XyuXly6DGyFbAaOtaDqF9DA9oM1lNQJIogkATrAaUHdM6wmu-wQFPdm7S2z3QHF-ct9BRpUqZBljd3HEc0KE8kLK91stBoAm3-uUq2p-OjPqfkbEvB4fhAsa2vWVbs8_xQFlU-h_kCsyVSlQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Preparation of bifunctional fluorescence sensor and application of bifunctional fluorescence sensor in SARS-CoV-2 Mpro detection and inhibitor screening</title><source>esp@cenet</source><creator>YU XIEAN ; WANG PING ; WANG BING ; HAN LEI ; LIN JIAOLI ; LI MEIFANG ; TIAN JIANGWEI</creator><creatorcontrib>YU XIEAN ; WANG PING ; WANG BING ; HAN LEI ; LIN JIAOLI ; LI MEIFANG ; TIAN JIANGWEI</creatorcontrib><description>The invention discloses preparation of a difunctional fluorescence sensor and application of the difunctional fluorescence sensor in SARS-CoV-2Mpro detection and inhibitor screening, and the preparation comprises the following steps: dissolving an AIE probe TPE-Ph-In and a substrate S-TLG in a THF-H2O solution, fully and uniformly mixing, adding glutaraldehyde, and stirring for reaction under closed and dark conditions; the fluorescence sensor is obtained after the reaction is completed. The sensor disclosed by the invention is simple to prepare, mild in reaction condition, convenient to operate and easy to prepare in batches, and a response result can be obtained within 45 minutes after the constructed sensor is added into SARS-CoV-2Mpro to be incubated: after the anti-Mpro active substance is added, different fluorescence signals are generated and can reflect the strength of the anti-Mpro activity, so that the sensitivity of the anti-Mpro activity is improved. And a new tool and a new method are provided fo</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20240823&amp;DB=EPODOC&amp;CC=CN&amp;NR=118533798A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20240823&amp;DB=EPODOC&amp;CC=CN&amp;NR=118533798A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YU XIEAN</creatorcontrib><creatorcontrib>WANG PING</creatorcontrib><creatorcontrib>WANG BING</creatorcontrib><creatorcontrib>HAN LEI</creatorcontrib><creatorcontrib>LIN JIAOLI</creatorcontrib><creatorcontrib>LI MEIFANG</creatorcontrib><creatorcontrib>TIAN JIANGWEI</creatorcontrib><title>Preparation of bifunctional fluorescence sensor and application of bifunctional fluorescence sensor in SARS-CoV-2 Mpro detection and inhibitor screening</title><description>The invention discloses preparation of a difunctional fluorescence sensor and application of the difunctional fluorescence sensor in SARS-CoV-2Mpro detection and inhibitor screening, and the preparation comprises the following steps: dissolving an AIE probe TPE-Ph-In and a substrate S-TLG in a THF-H2O solution, fully and uniformly mixing, adding glutaraldehyde, and stirring for reaction under closed and dark conditions; the fluorescence sensor is obtained after the reaction is completed. The sensor disclosed by the invention is simple to prepare, mild in reaction condition, convenient to operate and easy to prepare in batches, and a response result can be obtained within 45 minutes after the constructed sensor is added into SARS-CoV-2Mpro to be incubated: after the anti-Mpro active substance is added, different fluorescence signals are generated and can reflect the strength of the anti-Mpro activity, so that the sensitivity of the anti-Mpro activity is improved. And a new tool and a new method are provided fo</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjUEKwjAQRbtxIeodxgN0UYvYLktR3ChixW2ZphMdCJOQpHfxuNbiAbr6fHjv_2XyuXly6DGyFbAaOtaDqF9DA9oM1lNQJIogkATrAaUHdM6wmu-wQFPdm7S2z3QHF-ct9BRpUqZBljd3HEc0KE8kLK91stBoAm3-uUq2p-OjPqfkbEvB4fhAsa2vWVbs8_xQFlU-h_kCsyVSlQ</recordid><startdate>20240823</startdate><enddate>20240823</enddate><creator>YU XIEAN</creator><creator>WANG PING</creator><creator>WANG BING</creator><creator>HAN LEI</creator><creator>LIN JIAOLI</creator><creator>LI MEIFANG</creator><creator>TIAN JIANGWEI</creator><scope>EVB</scope></search><sort><creationdate>20240823</creationdate><title>Preparation of bifunctional fluorescence sensor and application of bifunctional fluorescence sensor in SARS-CoV-2 Mpro detection and inhibitor screening</title><author>YU XIEAN ; WANG PING ; WANG BING ; HAN LEI ; LIN JIAOLI ; LI MEIFANG ; TIAN JIANGWEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118533798A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>YU XIEAN</creatorcontrib><creatorcontrib>WANG PING</creatorcontrib><creatorcontrib>WANG BING</creatorcontrib><creatorcontrib>HAN LEI</creatorcontrib><creatorcontrib>LIN JIAOLI</creatorcontrib><creatorcontrib>LI MEIFANG</creatorcontrib><creatorcontrib>TIAN JIANGWEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YU XIEAN</au><au>WANG PING</au><au>WANG BING</au><au>HAN LEI</au><au>LIN JIAOLI</au><au>LI MEIFANG</au><au>TIAN JIANGWEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Preparation of bifunctional fluorescence sensor and application of bifunctional fluorescence sensor in SARS-CoV-2 Mpro detection and inhibitor screening</title><date>2024-08-23</date><risdate>2024</risdate><abstract>The invention discloses preparation of a difunctional fluorescence sensor and application of the difunctional fluorescence sensor in SARS-CoV-2Mpro detection and inhibitor screening, and the preparation comprises the following steps: dissolving an AIE probe TPE-Ph-In and a substrate S-TLG in a THF-H2O solution, fully and uniformly mixing, adding glutaraldehyde, and stirring for reaction under closed and dark conditions; the fluorescence sensor is obtained after the reaction is completed. The sensor disclosed by the invention is simple to prepare, mild in reaction condition, convenient to operate and easy to prepare in batches, and a response result can be obtained within 45 minutes after the constructed sensor is added into SARS-CoV-2Mpro to be incubated: after the anti-Mpro active substance is added, different fluorescence signals are generated and can reflect the strength of the anti-Mpro activity, so that the sensitivity of the anti-Mpro activity is improved. And a new tool and a new method are provided fo</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN118533798A
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Preparation of bifunctional fluorescence sensor and application of bifunctional fluorescence sensor in SARS-CoV-2 Mpro detection and inhibitor screening
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T00%3A44%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=YU%20XIEAN&rft.date=2024-08-23&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN118533798A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true