Method for screening retrogression regulation receptor nuclear gene responding to rape cytoplasmic male sterility mitochondrial sterility gene

The invention discloses a method for screening a retrogression regulation receptor nuclear gene responding to a rape cytoplasmic male sterility mitochondrial sterility gene. The method comprises the following steps: cloning a rape cytoplasmic male sterility pollen abortion mitochondrial sterility gene; constructing a plant expression vector of a mitochondrial sterile gene, and carrying out genetic transformation on arabidopsis thaliana; a differential expression gene DEGs is obtained; calculating a co-expression relationship among the genes to obtain an expression regulation relationship and a regulation direction among the genes so as to construct a co-expression network diagram of the genes; selecting a candidate nuclear gene to construct a double-process homozygous arabidopsis thaliana mutant; and a nuclear gene capable of recovering the pollen fertility of the mutant is identified. The method solves the problem that the existing method cannot accurately identify the nuclear gene of the mitochondrial steri