Catalpa bungei CbuPDS gene VIGS silencing system and construction method thereof

The invention relates to the field of plant molecular biology, in particular to a catalpa bungei CbuPDS gene VIGS silencing system and a construction method thereof. According to the invention, tender leaves of catalpa bungei tissue culture seedlings, which are most easily obtained, are used as materials, and a gene silencing transformation system which is based on the tender leaves of the catalpa bungei tissue culture seedlings and can trigger a gene with relatively long duration is established. Agrobacterium carrying pTRV1 and agrobacterium carrying a pTRV2-CbuPDS gene silencing vector are used for dip-dyeing catalpa bungei through a leaf injection method, and newly grown leaves still have a photobleaching symptom after inoculation is carried out for 2 months. Real-time fluorescent quantitative PCR (polymerase chain reaction) verifies that the relative expression level of the CbuPDS gene of the leaves with photobleaching symptoms is greatly reduced. The leaves with photobleaching symptoms of catalpa bungei