Double-clamp-shaped three-chain helix complex, gene loop and application in preparation of CTCs-EMT phenotype analysis reagent

The invention relates to the technical field of tumor cell detection, in particular to a double-clamp-shaped three-chain helix complex, a gene loop and application in preparation of a CTCs-EMT phenotype analysis reagent. The double-clamp-shaped three-chain helix complex is constructed by a sequence 1, a sequence 2, a sequence 3 and a sequence 4; the nucleotide sequences of the sequences 1-4 are as shown in SEQ ID No. 1-4. The green fluorescence (MFICSV) is increased while the red fluorescence (MFIEpCAM) is reduced, which indicates that the expression of the intermediate marker CSV is increased while the expression of the epithelial marker EpCAM is reduced in the CTCs-EMT process. The expression conditions of the markers CSV and EpCAM are verified through WB and FCM based on antigen-antibody interaction detection, the result shows that the three methods have good consistency, and the reliability of the result of CTCs-EMT phenotype analysis of the double-pincer gene loop is proved. 本发明涉及肿瘤细胞检测技术领域，特别是涉及一种双钳状三链螺