Recombinant yeast engineering bacterium for producing tripterygium wilfordii Abietatrien-14-ol

The invention discloses a recombinant yeast engineering bacterium for producing tripterygium wilfordii Abietatrien-14-ol and a preparation method of the recombinant yeast engineering bacterium. The recombinant yeast engineering bacteria disclosed by the invention are subjected to overexpression: a c...

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Bibliographische Detailangaben
Hauptverfasser:	WANG XIAOLI, ZHAO XIAOMIN, ZHANG BIN, ZHU CHUANSHU, MA ZHIQING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a recombinant yeast engineering bacterium for producing tripterygium wilfordii Abietatrien-14-ol and a preparation method of the recombinant yeast engineering bacterium. The recombinant yeast engineering bacteria disclosed by the invention are subjected to overexpression: a coding gene BTS1 of podophyllum pyrophosphate synthetase, a coding gene ERG20 of FPP synthase, a coding gene SmMS of salvia miltiorrhiza protanshinone diene synthetase, a coding gene TwTPS9 of tripterygium wilfordii diterpene synthase, and a coding gene TwCYP82D274 of cytochrome enzyme. A cytochrome P450 oxidoreductase coding gene TwCPR3 can also be subjected to overexpression; furthermore, overexpression of a 3-hydroxy-3-methylglutaryl coenzyme A reductase coding gene tHMG1, a phosphoketolase coding gene PK and a phosphotransacetylase coding gene PTA can be realized. According to the method disclosed by the invention, the production of the Abietatrien-14-ol in the yeast is realized. 本发明公开了产雷公藤Abietatrien-14-ol的重组酵母