Chlorella pyrenoidosa strain preservation method
The invention discloses a long-term preservation method of chlorella pyrenoidosa algae species. The chlorella pyrenoidosa solution is preserved in a two-phase protective agent, the lower layer is a composite water phase, and the upper layer is a liquid paraffin layer oil phase. The composite water-p...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a long-term preservation method of chlorella pyrenoidosa algae species. The chlorella pyrenoidosa solution is preserved in a two-phase protective agent, the lower layer is a composite water phase, and the upper layer is a liquid paraffin layer oil phase. The composite water-phase protective agent comprises the following mixed protective agent components: glycerol, trehalose and trace inorganic salt elements. And performing low-temperature cryopreservation by adopting a gradient cooling method. And the survival rate of cells preserved for 3 years reaches more than 80%. The method is easy to operate, low in cost, long in preservation time, not prone to pollution, capable of well maintaining the activity of the chlorella pyrenoidosa and easy to resuscitate, and has important significance on experiments and production and preservation of chlorella pyrenoidosa algae species.
本发明公开了一种蛋白核小球藻藻种长期保藏方法。它将蛋白核小球藻藻液保存于两相保护剂中,下层为复合水相,上层为液体石蜡层油相。复合水相保护剂包含以下成分:甘油、海藻糖、微量无机盐元素的混合保护剂组分。采用梯度降温法低温冷冻保存。3年保藏 |
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