Rapid extraction method of acyl carnitine in inherited metabolic disease and mass spectrometric detection device

The invention provides a rapid extraction method of acylcarnitine, which comprises the following steps: mixing an acetonitrile solution containing trace trifluoroacetic acid and formic acid as an extracting agent to replace a freezing method to precipitate protein, and adopting a methanol aqueous solution as a diluent to replace a nitrogen blowing drying and redissolving process by a solvent dilution method to obtain the acylcarnitine. Extracting acyl carnitine in the biological sample; the invention also provides a mass spectrometric detection device for acyl carnitine, a high-voltage power supply is loaded, a strong electric field is formed around a solution to be detected, liquid drops of the solution to be detected are induced to form electrospray, the electrospray is directly introduced into an inlet of a mass spectrometer, and real-time monitoring of the solution to be detected is completed in the mass spectrometer; the rapid extraction method is combined with a mass spectrum detection device, the struc