Magnetic bead one-step method plasmid DNA automatic extraction kit
The invention relates to the technical field of biotechnology, in particular to a kit for automatically extracting plasmid DNA through a magnetic bead one-step method. According to the kit provided by the invention, a one-step method lysis solution is matched with a premixed enzyme solution, plasmid...
Gespeichert in:
Hauptverfasser: | , , , , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention relates to the technical field of biotechnology, in particular to a kit for automatically extracting plasmid DNA through a magnetic bead one-step method. According to the kit provided by the invention, a one-step method lysis solution is matched with a premixed enzyme solution, plasmids can be rapidly split in one step, and the carboxyl modified magnetic beads have very strong affinity to plasmid DNA in an optimized binding enhancer environment. Protein impurities and combined genome DNA are cleaned away through the deproteinization liquid, residual salt ions are washed away through the rinsing liquid, and when conditions are changed, the magnetic beads release adsorbed plasmid DNA, so that the purpose of rapidly separating and purifying the plasmid DNA can be achieved, and the purity of the extracted plasmid is ensured. The solution in the kit can be pre-packaged in the reagent strip consumable, a sample only needs one-step simple pretreatment, extraction of plasmid DNA can be completed through |
---|