Freeze-drying protective agent for pseudomonas proteinans as well as preparation method and application of freeze-drying protective agent

The invention relates to the technical field of microorganisms, in particular to a freeze-drying protective agent for pseudomonas proteinans as well as a preparation method and application of the freeze-drying protective agent. The freeze-drying protective agent comprises the following components in...

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Bibliographische Detailangaben
Hauptverfasser: CHEN XINHUA, TAN PAN, ZHANG WEINI, YANG JINMEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of microorganisms, in particular to a freeze-drying protective agent for pseudomonas proteinans as well as a preparation method and application of the freeze-drying protective agent. The freeze-drying protective agent comprises the following components in percentage by mass: 5%-7.5% of lactose, 0-5% of mannitol, 0-5% of trehalose, 10%-15% of skimmed milk powder, 0-0.5% of vitamin C, 0-0.5% of gelatin, 0-3% of sorbitol, 0-1% of glycerol, 0-2% of L-sodium ascorbate and the balance of a sterilized 1wt% sodium chloride solution. Preferably, the freeze-drying protective agent comprises the following components in percentage by mass: 7.5% of lactose, 5% of mannitol, 5% of trehalose, 1% of glycerol, 10% of skimmed milk powder, 0.5% of vitamin C, 0.5% of gelatin and the balance of a sterilized 1wt% sodium chloride solution. The pseudomonas proteinans freeze-dried powder prepared by using the freeze-drying protective agent is stored for half a year at-80 DEG C, and the surv