Stable cTnI-C compound and preparation and application thereof

The invention provides a stable cTnI-C compound as well as preparation and application thereof. The cTnI-C compound is a conjugate of which the amino acid sequence is SEQ ID NO: 1 and the amino acid sequence is SEQ ID NO: 2. The cTnI-C compound is a conjugate of 24-110 amino acid SEQ ID NO: 1 and Tn...

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Hauptverfasser:	ZHANG YONGLEI, LI YANGXIN, ZHANG JIAO, YANG PINGPING, YANG ZHIWEI, HUANG FENFEI, YAO RONGXING, ZHOU BIN, LI MENGMENG, HUANG FEIHONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a stable cTnI-C compound as well as preparation and application thereof. The cTnI-C compound is a conjugate of which the amino acid sequence is SEQ ID NO: 1 and the amino acid sequence is SEQ ID NO: 2. The cTnI-C compound is a conjugate of 24-110 amino acid SEQ ID NO: 1 and TnC full-length SEQ ID NO: 2 in the most stable region of a cTnI molecule, the cTnI fragment and TnC are co-expressed in the same escherichia coli system and naturally combined to form a natural compound, the form of the compound is basically consistent with that of a natural molecule, and the compound is stable and not easy to degrade. Therefore, the technical problem that cTnI is unstable in overall length and easy to degrade in the prior art can be solved. 本发明提供了一种稳定的cTnI-C复合物及其制备及应用。所述的cTnI-C复合物为氨基酸序列为SEQ ID NO:1与氨基酸序列为SEQ ID NO:2的结合物。所述的cTnI-C复合物是cTnI分子最稳定的区域为24-110氨基酸SEQ ID NO:1与TnC的全长SEQ ID NO:2的结合物，此cTnI片段与TnC在同一大肠杆菌系统中共表达并自然结合形成天然的复合物，此复合物与天然分子的形态基本一致，且复合物稳定，不易降解。从而可以解决现有技术中cTnI全长不稳定易降解的技术问题。