Method for coupling antibody oligonucleotides

The invention belongs to the technical field of biology, and particularly relates to a method for antibody oligonucleotide coupling. The method comprises the step of coupling an antibody and a sulfydryl modified oligonucleotide together by using a heterobifunctional cross-linking agent (E)-6-(4-(2-(methylsulfonyl) vinyl) phenoxy) hexanoic acid-N-succinimide ester. The present invention has found that the compounds of formula I are particularly suitable for use as heterobifunctional cross-linking agents to couple antibodies and oligonucleotides together, and more importantly, one-step coupling can be carried out without unwanted polymerization or self-coupling, which simplifies the operation and reduces the number of treatment of antibodies, thereby reducing the number of times of treatment of antibodies. Therefore, the risk of activity reduction caused by structure change of the antibody in the treatment process is avoided. 本发明属于生物技术领域，具体涉及一种用于抗体寡核苷酸偶联的方法。所述方法包括使用异型双功能交联剂(E)-6-(4-(2-(甲基磺酰基)乙烯基)苯氧基)己酸-N-琥珀酰亚胺酯