Nested PCR (polymerase chain reaction) primer for identifying pseudoalteromonas and application of nested PCR primer

The invention belongs to the technical field of biology, and particularly relates to a nested PCR primer for identifying pseudoalteromonas and application of the nested PCR primer. According to the invention, a group of nested PCR amplification primers are designed based on a Pseudoalteromonas specific fragment TIR gene sequence. And a specific 469bp band is obtained through nested PCR (Polymerase Chain Reaction) amplification. The specific detection primer and the related detection method can be applied to rapid detection of pseudoalteromonas, have the advantages of high accuracy, strong specificity, high sensitivity, simple and rapid operation, short detection time, low detection cost and the like, have the detection limit of 100 pg/mu L, provide early diagnosis for pseudoalteromonas infection, and have very good application prospects. 本发明属于生物技术领域，具体涉及一种用于鉴定假交替单胞菌的巢式PCR引物及其应用。本发明基于假交替单胞菌特异性片段TIR基因序列设计了一组巢氏PCR扩增引物。通过巢氏PCR扩增得到特异性的469bp条带。该特异性检测引物及相关检测方法可应用于假交替单胞菌的快速检测，具有准确性高、特异性强、灵敏度高、操作简便快速、检测时间短、检测成本低等优点，检测