Culture medium for promoting somatic cell reprogramming and induction method

The invention provides a culture medium for promoting somatic cell reprogramming and an induction method. The culture medium contains growth factors with specific concentrations, reprogramming induction factors and extracellular matrix components, and can effectively promote reprogramming of somatic...

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Bibliographische Detailangaben
Hauptverfasser: ZHAN ZHENJIAN, CAI QIUYU, ZHENG SHIXIN, LI JUN, MENG YANFEN, LI YAORAO, QI GUOGUANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a culture medium for promoting somatic cell reprogramming and an induction method. The culture medium contains growth factors with specific concentrations, reprogramming induction factors and extracellular matrix components, and can effectively promote reprogramming of somatic cells, so that the reprogramming efficiency is remarkably improved. The induction method provided by the invention comprises the step of inoculating somatic cells into the culture medium for induction so as to obtain reprogrammed cells. The culture medium and the method provided by the invention have the advantages of high reprogramming efficiency, good safety, simplicity and convenience in operation and the like, and can be widely applied to stem cell production and research. 本发明提供了一种促进体细胞重编程的培养基及诱导方法。该培养基含有特定浓度的生长因子、重编程诱导因子和细胞外基质成分,能够有效地促进体细胞的重编程,从而显著提高重编程效率。本发明诱导方法包括将体细胞接种于该培养基中进行诱导,从而获得重编程细胞。本发明提供的培养基和方法具有重编程效率高、安全性好、操作简便等优点,可广泛应用于干细胞生产和研究。