Bamboo sweet potato tissue culture and rapid propagation method with bamboo sweet potato tubers as explants

The invention discloses a bamboo potato tissue culture and rapid propagation method taking a bamboo potato tuber as an explant, which comprises the following steps: selecting the bamboo potato tuber as the explant, and disinfecting; cutting the disinfected explant into small blocks, inoculating the...

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Bibliographische Detailangaben
Hauptverfasser: ZHOU RIHUA, GAO HONGMAO, LIN HAIPENG, TANG YUEDONG, LEE GWI WOO
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a bamboo potato tissue culture and rapid propagation method taking a bamboo potato tuber as an explant, which comprises the following steps: selecting the bamboo potato tuber as the explant, and disinfecting; cutting the disinfected explant into small blocks, inoculating the small blocks into an induction culture medium, and carrying out cluster bud induction culture; inoculating the bamboo potato cluster buds obtained by induction into a proliferation culture medium for proliferation culture; and inoculating the bamboo potato tissue culture seedlings obtained by multiplication culture into a rooting culture medium for rooting culture to obtain rooted seedlings. According to the method, tubers of the bamboo potatoes serve as explants, bamboo potato seedlings are produced through an in-vitro rapid propagation method, high-quality bamboo potato seedlings can be rapidly, effectively and massively produced, the method is suitable for industrialized production of the seedlings, large-scale