Method for identifying genetic relationship of hemibarbus amabilis
The invention belongs to the field of animal molecular genetics, and relates to a method for identifying the genetic relationship of amorphophallus dipsacus, which comprises the following steps: 1) extracting DNA (Deoxyribonucleic Acid) of amorphophallus dipsacus to be identified; (2) carrying out P...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention belongs to the field of animal molecular genetics, and relates to a method for identifying the genetic relationship of amorphophallus dipsacus, which comprises the following steps: 1) extracting DNA (Deoxyribonucleic Acid) of amorphophallus dipsacus to be identified; (2) carrying out PCR (Polymerase Chain Reaction) amplification by taking the extracted DNA as a template and taking a microsatellite primer pair of a microsatellite primer group for identifying the hemibarbus dipsacus as a primer, so as to obtain a PCR amplification product; (3) carrying out electrophoretic separation on a PCR amplification product on polyacrylamide gel; the method comprises the steps of (1) carrying out electrophoresis on the to-be-identified hemibarbus dimaculatus, (2) carrying out electrophoresis on the to-be-identified hemibarbus dimaculatus, (3) carrying out electrophoresis on the to-be-identified hemibarbus dimaculatus, (4) carrying out statistics on the genotype of the to-be-identified hemibarbus dimaculatus |
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