Biosensor for detecting zearalenone by activating CRISPR/Cas12a (clustered regularly interspaced short palindromic repeats/CRISPR associated 12a) through primer exchange reaction

The invention belongs to the technical field of biosensors, and provides a biosensor for detecting a primer exchange reaction activated CRISPR/Cas12a system of zearalenone, the biosensor comprises a composite structure Apt-T, a dumbbell structure, a primer chain Primer, a hairpin HP1, a hairpin HP2, a Cas12a protein, a double-chain complex, a reporter probe FQ chain, BST polymerase and dNTPs; the Apt-T composite probe is obtained by hybridizing an aptamer Apt and a T chain; the Apt, the T chain, the dumbbell structure, the primer chain Primer, the HP1, the HP2, the double-chain complex and the reporter probe FQ chain are as shown in SEQ ID NO: 1-8. The sensor disclosed by the invention has the advantages of high detection efficiency, sensitivity, strong selectivity and the like, can make up the defects and deficiencies of the existing zearalenone detection method, and realizes rapid and accurate quantitative detection of zearalenone. 本发明属于生物传感器技术领域，提供了一种检测玉米赤霉烯酮基于引物交换反应激活的CRISPR/Cas12a系统的生物传感器，包括复合结构Apt-T，哑铃结