Preparation method of Vutrisiran

The invention provides a preparation method of Vutrisiran (Vutrisiran). The Vutrisiran is a double-stranded RNA (Ribonucleic Acid) consisting of a positive-sense strand and an antisense strand which are complementarily paired; the preparation method comprises the following steps: mixing a positive-sense strand substrate fragment, an antisense strand substrate fragment and RNA ligase, the positive-sense strand substrate fragment being capable of forming a positive-sense strand, and the antisense strand substrate fragment being capable of forming an antisense strand; the positive-sense strand substrate fragment and the antisense strand substrate fragment are connected through a hydrogen bond formed by complementary bases, and the head and tail bases of the positive-sense strand substrate fragment and the antisense strand substrate fragment are not connected with each other, so that a double-strand nucleotide structure containing notches is formed; the method comprises the following steps: connecting basic group