Double-stranded RNA (Ribonucleic Acid) molecule as well as preparation method and application thereof

The invention discloses a double-stranded RNA (Ribonucleic Acid) molecule as well as a preparation method and application thereof, and belongs to the technical field of rice blast prevention and control. The method solves the problems that the existing rice blast prevention and control technology is easy to cause drug resistance of rice plants, is poor in environmental protection property and is relatively high in labor cost. The nucleotide sequence of the double-stranded RNA molecule is as shown in SEQ ID NO. 6; the preparation method comprises the following steps: 1, selecting a magnaporthe oryzae RHO1 gene target, and designing a primer; 2, extracting total DNA of the magnaporthe oryzae; 3, taking the total DNA of the magnaporthe oryzae as a template, carrying out PCR (Polymerase Chain Reaction) by adopting the primer obtained in the step 1 to obtain a magnaporthe oryzae RHO1 gene product, and purifying; and 4, carrying out in-vitro transcription on the purified magnaporthe oryzae RHO1 gene product to obta