Fermentation medium for producing marine low-temperature (-) gamma-lactamase strain

Fermentation medium for producing marine low-temperature (-) gamma-lactamase strain

The invention belongs to the technical field of bioengineering, and discloses a fermentation medium for producing marine low-temperature (-) gamma-lactamase strains. The culture medium is prepared from yeast extract, magnesium sulfate, soluble starch, KH2PO4, Na2HPO4, CaCl2, FeSO4 and NH4Cl. By adop...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: TONG XINYU, CHI XUEMEI, CHI NAIYU, XIA SHUIYING, CHEN WENLI, ZHANG QINGFANG
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention belongs to the technical field of bioengineering, and discloses a fermentation medium for producing marine low-temperature (-) gamma-lactamase strains. The culture medium is prepared from yeast extract, magnesium sulfate, soluble starch, KH2PO4, Na2HPO4, CaCl2, FeSO4 and NH4Cl. By adopting the fermentation culture medium, the enzyme activity of (-) gamma-lactamase can be remarkably improved, the resolution efficiency of (+/-) gamma-lactam is improved, the optical purity of a product is improved, and the enzyme activity is improved by 2.28 times compared with that before the culture medium is adopted. 本发明属于生物工程技术领域，公开了一种产海洋低温(-)γ-内酰胺酶菌株的发酵培养基。培养基包括酵母膏、硫酸镁、可溶性淀粉、KH2PO4、Na2HPO4、CaCl2、FeSO4、NH4Cl。采用该发酵培养基能够显著提升(-)γ-内酰胺酶酶活力，提高(±)γ-内酰胺的拆分效率，提升产物的光学纯度，酶活力较为进行培养基前提升2.28倍。