Sheep semen refrigerating fluid as well as preparation method and application thereof

The invention relates to the technical field of livestock semen cryopreservation, in particular to sheep semen refrigerating fluid as well as a preparation method and application thereof. The sheep semen refrigerating fluid is prepared from glucose, lactose, trisodium citrate, citric acid, tromethamine, sodium pyruvate, vitamin C, penicillin, streptomycin and fresh yolk. The cleaning agent further comprises ergothioneine, mitoxantrone mesylate, coconut oil monoethanolamide, lauryl sodium sulfate and glycerin. The invention also discloses a method for freezing fresh sheep semen by using the sheep semen freezing liquid and an unfreezing method of frozen sheep semen. The efficient antioxidant is added, the vitality of frozen sperms of the sheep semen is improved to 70% or above, the acrosome intact rate and the plasma membrane intact rate both reach 85% or above, the malformation rate is smaller than 15%, the actual production requirements can be met, and a reliable basis is provided for application and populari