Method for cultivating salt-tolerant miscanthus mutants based on 60Co-gamma radiation mutagenesis

The invention belongs to the technical field of agricultural planting, and particularly relates to a method for cultivating a salt-tolerant miscanthus sinensis mutant based on 60Co-gamma radiation mutagenesis, which comprises the following steps: S1, disinfecting a seed material: drying miscanthus s...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG YUN, XIE CUNCUN, JI SHUREN, YANG BAOLING, ZHAO RUIJIE, DONG CHENFEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of agricultural planting, and particularly relates to a method for cultivating a salt-tolerant miscanthus sinensis mutant based on 60Co-gamma radiation mutagenesis, which comprises the following steps: S1, disinfecting a seed material: drying miscanthus sinensis seeds, soaking, washing, adding a disinfectant for soaking and disinfecting, and washing and dewatering after disinfecting to obtain a sterile seed material; s2, radiation mutagenesis: inoculating the sterile seed material to a germination culture medium, selecting < 60 > Co-gamma radiation to induce mutation of the seed material, and transferring the mutated seed material into a screening culture medium to screen and cultivate germinated salt-tolerant miscanthus sinensis seedlings; s3, miscanthus sinensis seedling culture: transferring salt-tolerant miscanthus sinensis seedlings into a strong seedling culture medium for culture, and transplanting after seedling bud points grow out; s4, transplanting miscan