Universal primer for 18S rRNA gene sequencing

The invention discloses a universal primer for 18S rRNA (ribosomal Ribonucleic Acid) gene sequencing. And the universal primers are as follows: 415F: 5 '-GCAGGCGCANAHTTDCCCCA-3', and 2068R: 5 '-GGTTCMCCTACVRHWRCCTTGTTAYGAC-3', and the universal primers are as follows: 415F: 5 '-GCAGGCGCANAHTTDCCCCA-3', 2068R: 5 '- The 18S rR NA gene three-generation sequencing universal primer has the advantages that firstly, the 18S rR NA gene three-generation sequencing universal primer with wider types of amplified eukaryotes and longer amplification product fragments is designed, so that the research on the diversity of the eukaryotes can be deeply carried out; and 2, an M13 universal primer sequence is added on the primer, the M13 universal primer can be directly used for Sanger sequencing, the improvement is suitable for identification of eukaryotes, especially has a remarkable effect on rapid and accurate identification of pathogenic flagellates, such as giardia, trichomonad and the like, and is beneficial for improvin