Periodontal ligament stem cell proliferation culture medium and proliferation culture method thereof

The invention discloses a periodontal ligament stem cell proliferation culture medium and a proliferation culture method thereof in the field of cell culture. The periodontal ligament stem cell proliferation culture medium comprises dental pulp, tanshinone IIA, a low-sugar basic culture medium and a plurality of serum-free culture mediums, cutting the dental pulp into pieces, and adding type I collagenase into the dental pulp to obtain tissue blocks; inoculating the cells digested by the type I collagenase into another serum-free culture medium for culturing; adding tanshinone IIA into the serum-free culture medium to obtain a mixed culture medium, absorbing and abandoning the serum-free culture medium when the cells are cultured to the third generation and reach 80% fusion, and transferring the cells into a low-sugar basic culture medium for culture; after 48 hours, carrying out centrifugal treatment on the low-sugar basic culture medium, and leading out supernate; the periodontal ligament stem cell prolifer