Antibacterial peptide cecropin A mutant, recombinant expression vector, bacillus subtilis engineering bacteria and application

The invention discloses an antibacterial peptide cecropin A mutant, a recombinant expression vector, bacillus subtilis engineering bacteria and application. According to the invention, antibacterial peptide cecropin A is modified, a mutant with antibacterial activity superior to that of original cecropin A is obtained through screening, and heterologous expression is carried out by constructing a recombinant expression vector and adopting a biosafety strain bacillus subtilis. Compared with the original antibacterial peptide cecropin A, the antibacterial activity of the mutant provided by the invention is respectively improved by 60% and 80%. The bacillus subtilis is used as a secretory expression system to express the antibacterial peptide cecropin A mutant, industrial mass production is facilitated, and the cost is saved. 本发明公开了一种抗菌肽天蚕素A突变体、重组表达载体、枯草芽孢杆菌工程菌和应用。本发明通过对抗菌肽天蚕素A进行改造，筛选得到了抑菌活性优于原始天蚕素A的突变体，并通过构建重组表达载体，采用生物安全菌株枯草芽孢杆菌进行异源表达。本发明的突变体相对于原始抗菌肽天蚕素A，其抑菌活性分别提高了60%和80%。本发明利用枯草芽孢杆菌作为分泌表达系统表达抗菌肽天蚕素A突变体，利于工业化大量