Construction method and application of Duchenne muscular dystrophy monkey model

The invention provides a construction method and application of a Duchenne muscular dystrophy monkey model. The construction method comprises the following steps: designing sgRNA (small guide ribonucleic acid) aiming at a 50 # exon of a DMD gene; the mRNA mixture of spCas9 and sgRNA is injected into the fertilized ovum of the monkey, and the fertilized ovum after injection is transplanted into the uterus of the female monkey. According to the method, efficient sgRNA is obtained through screening, then the injection dosage is optimized, so that a male semi-zygote or female homozygote DMD model monkey can be obtained in the F0 generation, the time cost is greatly reduced, an ideal animal model can be obtained within a short time, and the method is suitable for large-scale popularization and application. And a reliable non-human primate DMD model is provided for researching the DMD and evaluating the effectiveness and safety of a DMD treatment method or medicine. 本发明提供了一种杜氏肌营养不良症猴模型的构建方法及其用途，其构建方法为：针对DMD基因50号外显子