Kit for simultaneously detecting basophilic granulocytes, eosinophilic granulocytes and neutrophil in human blood, detection method and application

The invention relates to the technical field of flow cytometry detection, and particularly discloses a kit for simultaneously detecting basophilic granulocytes, eosinophilic granulocytes and neutrophil in human blood as well as a detection method and application of the kit, and the kit comprises the following components: dead cell dye, an FcR blocking agent, fluorescently-labeled anti-human CD193 and CD63 antibodies, red blood cell lysis buffer and washing buffer. During detection, firstly, dead cell dye and an FcR blocking agent are added into whole blood, then fluorescent antibody staining is performed, and after erythrocytes are split, the proportion, the number and the degranulation level of basophilic granulocytes, eosinophilic granulocytes and neutrophil can be simultaneously detected by a flow cytometer. According to the kit and the detection method, the proportion, the number and the degranulation level of basophilic granulocytes, eosinophilic granulocytes and neutrophile granulocytes in human blood c