Kit and method for label-free visual detection of monkey pox virus based on CRISPR/Cas12j2

The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12j2-based label-free visual detection system for detecting a monkey pox virus. The CRISPR/Cas12j2-based label-free visual detection system comprises an RPA (recombinase polymerase amplification) primer combination and a CRISPR/Cas12j2 detection system, the primer group for amplifying the monkey pox virus comprises a primer group for amplifying specific fragments on J2R and F3L/F4L genes; the CRISPR/Cas12j2 detection system comprises a Cas12j2 protein, crRNA (Ribonucleic Acid) of a targeted monkey pox virus and a reporter probe. The invention further provides a corresponding detection method, the specific recognition characteristic of CRISPR/Cas12j2 on nucleic acid is combined with the characteristic of rapid target fragment amplification of RPA, large detection instruments and equipment are not needed, detection can be completed under the constant-temperature condition, the detection time is short, operation is eas