Serum biomarker for detecting colorectal cancer, SERS (Surface Enhanced Raman Scattering) sensor and preparation method

The invention relates to the technical field of biomedicine, in particular to a related serum biomarker for detecting colorectal cancer, an SERS (Surface Enhanced Raman Scattering) sensor and a preparation method. The SERS sensor is formed by a double-chain nucleic acid structure which is formed by...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN FENGSONG, YAO JIE, XU MIAOWEN, ZU WEIBING, CAO XIAOWEI, QIN SHICHEN, SHENG JINXIN, LI WEI, ZHU FENG, WANG XIAOYONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of biomedicine, in particular to a related serum biomarker for detecting colorectal cancer, an SERS (Surface Enhanced Raman Scattering) sensor and a preparation method. The SERS sensor is formed by a double-chain nucleic acid structure which is formed by hairpin structure DNA and single-chain DNA, wherein the hairpin structure DNA is marked by Raman signal molecules and specifically combined with related serum biomarkers, and the single-chain DNA is partially complementary with the hairpin structure DNA, and the SERS sensor is obtained according to the positions and intensities of characteristic peaks of 5-FAM and Cy5 at 1170 cm and 1346 cm . The rapid, high-sensitivity and synchronous detection of miR-21 and miR-122 in the serum can be realized; the SERS sensor disclosed by the invention has good specificity, stability and reproducibility, is simple to operate when being used for detecting a clinical serum sample, and can complete the detection of miR-2