Preparation method of primer for amplifying target nucleic acid

The invention relates to a preparation method of a primer for amplifying target nucleic acid, which comprises the following steps: providing a PCR template, a preparation primer 1 and a preparation primer 2, the PCR template comprises a 5p flank, a spacer primer and a 3p flank, the sequence of the preparation primer 1 is basically the same as the sequence of the 5p flank, and the sequence of the preparation primer 2 is basically complementary with the sequence of the 3p flank; carrying out PCR (Polymerase Chain Reaction) amplification to generate a double-chain precursor; wherein the preparation primer 2 can be digested, and a digested precursor is obtained after digestion; and further removing the negative chain to obtain the primer for amplifying the target nucleic acid. 本公开涉及一种扩增靶核酸的引物的制备方法，包括：提供PCR模板、制备引物1和制备引物2，所述PCR模板包括5p侧翼，间隔区引物和3p侧翼，所述制备引物1序列与5p侧翼序列基本相同，所述制备引物2序列与3p侧翼的序列基本上是互补的；进行PCR扩增，产生双链前体；其中制备引物2可被消化，经消化后得到消化的前体；进一步移除负链得到扩增靶核酸的引物。