Crypthecodinium cohnii endogenous promoter and application thereof
The invention relates to the technical field of biology, in particular to a crypthecodinium cohnii endogenous promoter and application thereof. In massive transcriptome data, a gene with a high expression level is mined, a promoter sequence of the gene is obtained through a chromosome walking method...
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| creator | ZHANG XIAO ZOU LIGONG YAO YITONG LI HONGYE |
| description | The invention relates to the technical field of biology, in particular to a crypthecodinium cohnii endogenous promoter and application thereof. In massive transcriptome data, a gene with a high expression level is mined, a promoter sequence of the gene is obtained through a chromosome walking method, the obtained promoter sequence is amplified and connected with a hygromycin gene, a transformation plasmid is constructed and introduced into crypthecodinium cohnii, a successful transformation strain is screened through hygromycin, and the crypthecodinium cohnii with the high expression level is obtained. And the effective promoter is obtained.
本发明涉及生物技术领域，特别涉及一种寇氏隐甲藻内源启动子及其应用。本发明在海量的转录组数据中，挖掘出高表达水平的基因，并通过染色体步移法获取所述基因的启动子序列，对获得的启动子序列进行扩增，并与潮霉素基因连接，构建转化质粒，将转化质粒导入寇氏隐甲藻，并通过潮霉素筛选成功的转化株，获得了有效启动子。 |
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本发明涉及生物技术领域，特别涉及一种寇氏隐甲藻内源启动子及其应用。本发明在海量的转录组数据中，挖掘出高表达水平的基因，并通过染色体步移法获取所述基因的启动子序列，对获得的启动子序列进行扩增，并与潮霉素基因连接，构建转化质粒，将转化质粒导入寇氏隐甲藻，并通过潮霉素筛选成功的转化株，获得了有效启动子。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240802&DB=EPODOC&CC=CN&NR=118421632A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240802&DB=EPODOC&CC=CN&NR=118421632A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG XIAO</creatorcontrib><creatorcontrib>ZOU LIGONG</creatorcontrib><creatorcontrib>YAO YITONG</creatorcontrib><creatorcontrib>LI HONGYE</creatorcontrib><title>Crypthecodinium cohnii endogenous promoter and application thereof</title><description>The invention relates to the technical field of biology, in particular to a crypthecodinium cohnii endogenous promoter and application thereof. In massive transcriptome data, a gene with a high expression level is mined, a promoter sequence of the gene is obtained through a chromosome walking method, the obtained promoter sequence is amplified and connected with a hygromycin gene, a transformation plasmid is constructed and introduced into crypthecodinium cohnii, a successful transformation strain is screened through hygromycin, and the crypthecodinium cohnii with the high expression level is obtained. And the effective promoter is obtained.
本发明涉及生物技术领域，特别涉及一种寇氏隐甲藻内源启动子及其应用。本发明在海量的转录组数据中，挖掘出高表达水平的基因，并通过染色体步移法获取所述基因的启动子序列，对获得的启动子序列进行扩增，并与潮霉素基因连接，构建转化质粒，将转化质粒导入寇氏隐甲藻，并通过潮霉素筛选成功的转化株，获得了有效启动子。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHByLqosKMlITc5PyczLLM1VSM7PyMvMVEjNS8lPT83LLy1WKCjKz80vSS1SSMxLUUgsKMjJTE4syczPUwBqK0rNT-NhYE1LzClO5YXS3AyKbq4hzh66qQX58anFBYnJqXmpJfHOfoaGFiZGhmbGRo7GxKgBALbvMvU</recordid><startdate>20240802</startdate><enddate>20240802</enddate><creator>ZHANG XIAO</creator><creator>ZOU LIGONG</creator><creator>YAO YITONG</creator><creator>LI HONGYE</creator><scope>EVB</scope></search><sort><creationdate>20240802</creationdate><title>Crypthecodinium cohnii endogenous promoter and application thereof</title><author>ZHANG XIAO ; ZOU LIGONG ; YAO YITONG ; LI HONGYE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118421632A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHANG XIAO</creatorcontrib><creatorcontrib>ZOU LIGONG</creatorcontrib><creatorcontrib>YAO YITONG</creatorcontrib><creatorcontrib>LI HONGYE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHANG XIAO</au><au>ZOU LIGONG</au><au>YAO YITONG</au><au>LI HONGYE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Crypthecodinium cohnii endogenous promoter and application thereof</title><date>2024-08-02</date><risdate>2024</risdate><abstract>The invention relates to the technical field of biology, in particular to a crypthecodinium cohnii endogenous promoter and application thereof. In massive transcriptome data, a gene with a high expression level is mined, a promoter sequence of the gene is obtained through a chromosome walking method, the obtained promoter sequence is amplified and connected with a hygromycin gene, a transformation plasmid is constructed and introduced into crypthecodinium cohnii, a successful transformation strain is screened through hygromycin, and the crypthecodinium cohnii with the high expression level is obtained. And the effective promoter is obtained.
本发明涉及生物技术领域，特别涉及一种寇氏隐甲藻内源启动子及其应用。本发明在海量的转录组数据中，挖掘出高表达水平的基因，并通过染色体步移法获取所述基因的启动子序列，对获得的启动子序列进行扩增，并与潮霉素基因连接，构建转化质粒，将转化质粒导入寇氏隐甲藻，并通过潮霉素筛选成功的转化株，获得了有效启动子。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Crypthecodinium cohnii endogenous promoter and application thereof |
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