Crypthecodinium cohnii endogenous promoter and application thereof

The invention relates to the technical field of biology, in particular to a crypthecodinium cohnii endogenous promoter and application thereof. In massive transcriptome data, a gene with a high expression level is mined, a promoter sequence of the gene is obtained through a chromosome walking method, the obtained promoter sequence is amplified and connected with a hygromycin gene, a transformation plasmid is constructed and introduced into crypthecodinium cohnii, a successful transformation strain is screened through hygromycin, and the crypthecodinium cohnii with the high expression level is obtained. And the effective promoter is obtained. 本发明涉及生物技术领域，特别涉及一种寇氏隐甲藻内源启动子及其应用。本发明在海量的转录组数据中，挖掘出高表达水平的基因，并通过染色体步移法获取所述基因的启动子序列，对获得的启动子序列进行扩增，并与潮霉素基因连接，构建转化质粒，将转化质粒导入寇氏隐甲藻，并通过潮霉素筛选成功的转化株，获得了有效启动子。