Chimeric depolymerases of multi-serotype klebsiella pneumoniae as well as preparation method and application of chimeric depolymerases

The invention is applicable to the technical field of bioengineering, and provides a chimeric depolymerases of multi-serotype klebsiella pneumoniae and a preparation method and application thereof, the amino acid sequence of the chimeric depolymerases is shown as SEQ ID NO.1 in a sequence table, and the nucleotide sequence of the coding gene of the chimeric depolymerases is shown as SEQ ID NO.2 in the sequence table. The chimeric depolymerization enzyme provided by the invention is novel in sequence, has better acid-base and high-temperature resistance in vitro, shows broad-spectrum activity to klebsiella pneumoniae of K1 and K2 serotypes, and can synchronously remove capsular polysaccharide of the klebsiella pneumoniae of the K1 and K2 serotypes and inhibit toxicity of the klebsiella pneumoniae of the K1 and K2 serotypes. The chimeric depolymerases are expected to become novel antibacterial drugs for preventing and treating klebsiella pneumoniae infection, and have good clinical application value. 本发明适用于生物工程