Method for dissociating blastocyst cells into single cells

The invention discloses a method for dissociating blastocyst cells into single cells, and relates to the technical field of genetic detection, in particular to a method for dissociating blastocyst cells into single cells, which comprises the following steps: taking abnormal blastocysts or chimera blastocysts donated by patients in genetic detection before implantation as experimental material blastocysts; the method for dissociating blastocyst cells into single cells comprises the following specific steps: step 1, blastocyst resuscitation; 2, an Accuase Solution solution is prepared, and the Accuase Solution solution is prepared; step 3, preparing an operation vessel; step 4, removing zona pellucida of the blastocyst; step 5, cell dissociation; step 6, dispersing cells; and 7, collecting single cells. The method for dissociating the blastocyst cells into the single cells is simple to operate and small in cell damage; according to the method, only one kind of enzyme Accutase Solution which is small in irritati