Pseudomonas high-efficiency fermentation culture medium and fermentation method and application thereof

The invention belongs to the field of microbial engineering, and discloses an efficient fermentation medium for pseudomonas as well as a fermentation method and application of the efficient fermentation medium. Comprising soluble starch, a nitrogen source, magnesium sulfate heptahydrate, dipotassium...

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Hauptverfasser: CHI NAIYU, ZHAO LU, YU SHUANG, WANG YAN, ZHANG QINGFANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of microbial engineering, and discloses an efficient fermentation medium for pseudomonas as well as a fermentation method and application of the efficient fermentation medium. Comprising soluble starch, a nitrogen source, magnesium sulfate heptahydrate, dipotassium phosphate, sodium chloride and potassium chloride. According to the application of the high-efficiency fermentation culture medium for the pseudomonas to culture of the pseudomonas Pseudomonas Y-4, the fermentation culture medium is inoculated with an activated seed solution of the Pseudomonas Pseudomonas Y-4 thallus, and the seed solution is subjected to shake culture for 48 hours under the conditions of 25 DEG C and 150 r/min. The inoculation amount of the Pseudomonasjaponica Y-4 is 1 vt%, and the liquid loading amount of the Pseudomonasjaponica Y-4 is 100 mL/250 mL. The enzyme activity can be effectively improved, and a foundation is laid for converting laboratory research results into large-scale industrial pr