Fragment consistency method for ultra-sensitive detection of abnormal methylation

Provided herein are methods related to detecting DNA methylation (e.g., methylation levels at the CpG dinucleotide cluster), as well as therapeutic methods, uses, systems, and computer readable storage media related thereto. These methods allow detection of abnormal DNA methylation patterns with low background and increased signal-to-noise ratio, which is particularly useful for early detection or monitoring of cancer. 本文提供了与检测DNA甲基化(例如，CpG二核苷酸簇处的甲基化水平)相关的方法，以及与其相关的治疗方法、用途、系统和计算机可读存储介质。这些方法允许以低背景和增加的信噪比进行对异常DNA甲基化模式的检测，这尤其可用于癌症的早期检测或监测。