Kit for combining dual immunohistochemistry with elastic fiber dyeing, dyeing method and application

The invention provides a kit for combining dual immunohistochemistry with elastic fiber dyeing, a dyeing method and application. The kit comprises a combined primary antibody, and the combined primary antibody comprises different species of anti-CK7 and CD34 monoclonal antibodies, such as a rabbit anti-human CK7 monoclonal antibody and a mouse anti-human CD34 monoclonal antibody. CK7 and CD34 are selected as a combined primary antibody, the growth mode of tumor epithelium can be accurately indicated, alveolar spacing fiber tissue hyperplasia and thickening caused by tumors and alveolar structure changes caused by other factors can be clearly seen, and by combining independent Victoria blue dyeing, the alveolar structure change caused by other factors can be accurately determined. The elastic fibers of arteriovenous walls and lung layer pleura can be clearly seen, and the method is used for judging the integrity of the elastic fibers on the lung surface and judging the tumor infiltration condition. By means of